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KMID : 0545119950050040224
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 4 p.224 ~ p.228
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Effect of Galactose Feeding Strategy on Heterolgous Human Lipocortin-I Production in the Fed-Batch Culture of Saccharomyces Cerevisiae Controlled by the GAL10 Promoter
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Chung, Bong Hyun
Kim, Byung Moon/Rhee, Sang Ki/Park, Young Hoon/Naw, Soo Wan
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Abstract
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Fed-batch fermentations were conducted to produce human lipocortin-I (LC1), a potential anti-inflammatory agent, from recombinant Sacchromyces cerevisiae carrying a galactose-inducible expression system. The cell growth, expression level of LC1, and the plasmid stability were investigated under various LC1 induction modes performed by three different galactose feeding strategies. Galactoe was fed to induce the expression of LC1 from the beginning (initial induction) of culture or when the cell concentration reached 120 OD (mid-phase induction) or 300 OD (late induction). Among the three galactose-induction modes tested, the initial induction mode yielded the best result with respect to a final expression level of LC1. Fedbatch fermentation with initial induction mode produced LC1 at a conentration of 220 ㎎/ℓ, which corresponded to 1.38- and 1.53-fold increases over those produced by mid-phase and late induction modes.
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